- Use a 27-gauge (or smaller) vitrectomy system
- Prime the vitrectomy system without fluid in the cutter line. If using the Alcon CONSTELLATION® vision system: The "Setup" screen is divided into 6 boxes. The top middle box says "Probe." In this box it says "Skip Prime". Push the button next to this and it will place a green check next to it. When everything on the machine is set up and ready to prime, just select "Start Prime" as normal. The whole system will prime like normal except there will be no fluid in the cutter line, allowing for a pure sample to be taken
- Setup the specimen collection. Separate the connectors on the blue aspiration tubing behind the vitrectomy cutter. A syringe can be directly attached to the aspiration line (and aspirated by an assistant during vitrectomy), but a more elegant technique is to attach a collection tube in a “closed-loop” system. Find the white adaptor from the pack contents and connect it to the blue connector closest to the vitrectomy cutter. Place a collection tube (e.g. for blood typing) in a sterile plastic bag and tie the end of the bag off to keep the setup sterile (alternatively, a sterile collection tube can be used). Insert two 19-gauge needles through the bag into the collection tube. Attach the ends of the separated aspiration line to the 19-gauge needles (Figure 19.3.5)
- Set-up as for a standard 3-port, 27-gauge vitrectomy procedure
- Consider elevating the intra-ocular pressure (IOP) if the tumor is prone to bleed
- Pass the vitrectomy cutter through the retina into the tumor, avoiding retinal vessels and rotating the cutter so that the port faces the tumor
- Start with maximum suction (650 mmHg) and a low cutting rate (100 - 200 cpm)
- Rotate the cutter to obtain the maximal volume of specimen (Figure 19.3.6)
- If hemorrhage occurs, raise the IOP until it stops. This is particularly prone to occur at the time the vitrector cutter is withdrawn from the tumor
- Remove the cutter from the tumor, increase the cutting rate to maximum and cut / aspirate a minimal amount of vitreous to flush the sample up the tube. This is also important to lower the IOP if it has been raised (since the infusion line will not have been cleared of vitreous, the IOP will not fall even if the infusion pressure is lowered). It is important to ensure that the central retinal artery is perfused prior to completing the case
- Withdraw the vitrectomy instruments from the eye
- Check the collection tube (or syringe) to ensure sufficient specimen has been collected
- If insufficient specimen has been collected, a second pass of the tumor can be performed, with the same or a new collection tube
- Once sufficient specimen has been obtained, the collection tube, bag and two 19-gauge needles can be removed from the setup and passed to an assistant or scout nurse. If necessary, transport medium (e.g. RPMI) can be added directly to the collection tube
- The specimen should be sent directly to the cytopathologist, on ice if necessary (discuss timing, transportation and media prior to the case)
- Remove the cannulae. It is not required to perform further vitrectomy or induce a PVD
- Apply triple freeze-thaw cryotherapy to the sclerotomies to minimize the risk of tumor seeding. Sclerotomy suturing is not usually necessary